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Product Info Summary
| SKU: | M00549 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-Wnt5a Rabbit Monoclonal Antibody
SKU/Catalog Number
M00549
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Wnt5a Rabbit Monoclonal Antibody catalog # M00549. Tested in WB, ICC/IF, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Wnt5a Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00549)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ABEE-23
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Wnt5a
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00549 is reactive to WNT5A in Human
Applications
M00549 is guaranteed for Flow Cytometry, IF, ICC, WB Boster Guarantee
Observed Molecular Weight
42 kDa, 48 kDa
Calculated molecular weight
42.339kDa
Background of Wnt-5a
Dynamin-related GTPase required for mitochondrial fusion and regulation of apoptosis. May form a diffusion barrier for proteins stored in mitochondrial cristae. Proteolytic processing in response to intrinsic apoptotic signals may lead to disassembly of OPA1 oligomers and release of the caspase activator cytochrome C (CYCS) into the mitochondrial intermembrane space.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:1000
ICC/IF 1:50-1:200
FC 1:20
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.
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Western blot analysis of Wnt5a expression in (1) HeLa cell lysate; (2) SK-OV-3 cell lysate.
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All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.
Protein Target Info & Infographic
Gene/Protein Information For WNT5A (Source: Uniprot.org, NCBI)
Gene Name
WNT5A
Full Name
Protein Wnt-5a
Weight
42.339kDa
Superfamily
Wnt family
Alternative Names
hWNT5A; protein Wnt-5a; wingless-type MMTV integration site family, member 5A; WNT-5A protein; Wnt5a; Wnt-5a WNT5A hWNT5A Wnt family member 5A protein Wnt-5a|WNT-5A protein|epididymis secretory sperm binding protein|wingless-type MMTV integration site family, member 5A
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on WNT5A, check out the WNT5A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for WNT5A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Wnt5a Rabbit Monoclonal Antibody (M00549)
Hello CJ!
No publications found for M00549
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Recommended Resources
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Customer Reviews
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*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
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Find answers in Q&As, reviews.
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17 Customer Q&As for Anti-Wnt5a Rabbit Monoclonal Antibody
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for endometrium using anti-Wnt5a Rabbit Monoclonal antibody M00549. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-04-17
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-17
Question
We have been able to see staining in human placenta. Do you have any suggestions? Is anti-Wnt5a Rabbit Monoclonal antibody supposed to stain placenta positively?
Verified Customer
Verified customer
Asked: 2020-03-16
Answer
Based on literature placenta does express WNT5A. Based on Uniprot.org, WNT5A is expressed in endometrium, mesangial cell tongue, placenta, among other tissues. Regarding which tissues have WNT5A expression, here are a few articles citing expression in various tissues:
Mesangial cell, and Tongue, Pubmed ID: 14702039
Placenta, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2020-03-16
Question
I would like to test anti-Wnt5a Rabbit Monoclonal antibody M00549 on human endometrium for research purposes, then I may be interested in using anti-Wnt5a Rabbit Monoclonal antibody M00549 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-02-10
Answer
The products we sell, including anti-Wnt5a Rabbit Monoclonal antibody M00549, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-02-10
Question
Here is the WB image, lot number and protocol we used for endometrium using anti-Wnt5a Rabbit Monoclonal antibody M00549. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-01-29
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-01-29
Question
Is this M00549 anti-Wnt5a Rabbit Monoclonal antibody reactive to the isotypes of WNT5A?
Verified Customer
Verified customer
Asked: 2020-01-08
Answer
The immunogen of M00549 anti-Wnt5a Rabbit Monoclonal antibody is A synthesized peptide derived from human Wnt5a. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-01-08
Question
Will anti-Wnt5a Rabbit Monoclonal antibody M00549 work on horse WB with mesangial cell tongue?
Verified Customer
Verified customer
Asked: 2019-12-30
Answer
Our lab technicians have not tested anti-Wnt5a Rabbit Monoclonal antibody M00549 on horse. You can run a BLAST between horse and the immunogen sequence of anti-Wnt5a Rabbit Monoclonal antibody M00549 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse mesangial cell tongue in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-30
Question
We are currently using anti-Wnt5a Rabbit Monoclonal antibody M00549 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on canine tissues as well?
E. Taylor
Verified customer
Asked: 2019-12-20
Answer
The anti-Wnt5a Rabbit Monoclonal antibody (M00549) has not been tested for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-20
Question
We have tried in the past anti-Wnt5a Rabbit Monoclonal antibody for WB on mesangial cell tongue in a previous experiment. I am using human, and I plan to use the antibody for IF next. I am interested in examining mesangial cell tongue as well as placenta in our next experiment. Could you please give me some suggestion on which antibody would work the best for IF?
Verified Customer
Verified customer
Asked: 2019-12-18
Answer
I looked at the website and datasheets of our anti-Wnt5a Rabbit Monoclonal antibody and it appears that M00549 has been tested on human in both WB and IF. Thus M00549 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-12-18
Question
I have a question about product M00549, anti-Wnt5a Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-06-28
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00549 anti-Wnt5a Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-06-28
Question
I see that the anti-Wnt5a Rabbit Monoclonal antibody M00549 works with WB, what is the protocol used to produce the result images on the product page?
C. Mitchell
Verified customer
Asked: 2019-01-18
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-01-18
Question
Do you have a BSA free version of anti-Wnt5a Rabbit Monoclonal antibody M00549 available?
Verified Customer
Verified customer
Asked: 2018-03-12
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Wnt5a Rabbit Monoclonal antibody M00549 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-03-12
Question
I was wanting to use your anti-Wnt5a Rabbit Monoclonal antibody for WB for human endometrium on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human endometrium identification?
Verified Customer
Verified customer
Asked: 2017-09-25
Answer
As indicated on the product datasheet, M00549 anti-Wnt5a Rabbit Monoclonal antibody has been validated for IF, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human endometrium in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-09-25
Question
Does anti-Wnt5a Rabbit Monoclonal antibody M00549 work for WB with endometrium?
Verified Customer
Verified customer
Asked: 2017-09-18
Answer
According to the expression profile of endometrium, WNT5A is highly expressed in endometrium. So, it is likely that anti-Wnt5a Rabbit Monoclonal antibody M00549 will work for WB with endometrium.
Boster Scientific Support
Answered: 2017-09-18
Question
I am interested in using your anti-Wnt5a Rabbit Monoclonal antibody for planar cell polarity pathway involved in outflow tract morphogenesis studies. Has this antibody been tested with western blotting on hela cell lysate? We would like to see some validation images before ordering.
N. Roberts
Verified customer
Asked: 2016-01-14
Answer
We appreciate your inquiry. This M00549 anti-Wnt5a Rabbit Monoclonal antibody is validated on hela cell lysate. It is guaranteed to work for IF, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2016-01-14
Question
Does M00549 anti-Wnt5a Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
B. Wu
Verified customer
Asked: 2013-12-17
Answer
It shows on the product datasheet, M00549 anti-Wnt5a Rabbit Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2013-12-17
Question
Is a blocking peptide available for product anti-Wnt5a Rabbit Monoclonal antibody (M00549)?
A. Li
Verified customer
Asked: 2013-09-20
Answer
We do provide the blocking peptide for product anti-Wnt5a Rabbit Monoclonal antibody (M00549). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2013-09-20
Question
We were satisfied with the WB result of your anti-Wnt5a Rabbit Monoclonal antibody. However we have seen positive staining in mesangial cell tongue extracellular using this antibody. Is that expected? Could you tell me where is WNT5A supposed to be expressed?
P. Krishna
Verified customer
Asked: 2013-06-26
Answer
From what I have seen in literature, mesangial cell tongue does express WNT5A. Generally WNT5A expresses in secreted, extracellular space, extracellular. Regarding which tissues have WNT5A expression, here are a few articles citing expression in various tissues:
Mesangial cell, and Tongue, Pubmed ID: 14702039
Placenta, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2013-06-26
